Validação de técnicas de coloração utilizadas em parasitologia.

Abstract

Introduction: The coloring of blades in parasitology is the main method of diagnosis and the most widely used dye is being regarded as lugol's gold standard in this kind of technique. Objective: to Compare the colors of Chlorosis and Ziehl-Neelsen Hematoxylin and verify the sensitivity and specificity of these colourings in relation to lugol's iodine. Materials and methods: analysis of 218 stool samples from the patients in the project prevention and detection of infectious diseases and parasitic diseases of ASCES College, Caruaru-PE. These samples were subjected to coloration techniques could be made, analysis of samples analysed. Results: of these, 19% (42/218) were positive in Lugol's iodine staining. To check the other colorings, observed greater positivity in permanent staining techniques, with 31.2% (68/218) were positive in iron Hematoxylin staining and 34% (76/218) positive Ziehl-Neelsen staining. Thus demonstrating an increase of positivity around 64.2% by iron hematoxylin and 78.9% by Ziehl-Neelsen staining. With 100% sensitivity in both techniques. Conclusion: it is concluded that the colors presented satisfactory results as the identification of protozoa by its high sensitivity (100%) both techniques can be used as screening methods.