Desenvolvimento e validação de um método para quantificação de minoxidil em soluções capilares por clae-uv

Abstract

Minoxidil is a vasodilator, originally used for the treatment of hypertension, but has been widely used for hair growth in people with baldness and growth or repair of the failed beard. Fast and gentle analytical methods for the quantification of minoxidil are quite valued in pharmaceutical quality control. In this context, the objective of this work was based on the development and validation of a new analytical method by Liquid High Efficiency Chromatography (HPLC) for quantification of the active principle minoxidil.The analyzes were carried out by a high performance liquid chromatograph Prominence Shimadzu, in isocratic mode using C8 column, with detection at 254nm. The mobile phase consisted of methanol and water at 0.5% acetic acid (35:65 v/v) at a flow rate of 1 mL/min. The linearity range of the method was 50 μg/mL to 150 μg/mL (R²=0.9929011).The retention time of minoxidil was ± 3.37 minutes, in this way, it proved to be a sensitive, specific, accurate, precise and fast method.The identification and quantification of minoxidil were carried out in six drugs purchased in pharmacies of manipulation of Caruaru - PE, where all the samples obtained results in the recommended range, making them approved.